Sybr Green Primer Design Tool

Applied Biosystems™ SYBR™ Green master mixes are designed for quantitative real-time PCR using a set of two PCR primers that flank the target region. The master mixes contain buffer, dNTPs, thermostable hot-start DNA polymerase, and of course, SYBR Green dye—everything needed for real-time PCR, except the sample and PCR primer pair.

Which SYBR Green master mix is right for you?

SYBR Green master mix formulations have improved over the years, as researchers have demanded improved sensitivity, greater specificity, enhanced reproducibility, faster time-to-results, and protection from contamination. Although there are now many choices of SYBR Green master mixes available, below are the recommended master mixes, by application.

Applications	Gene expression analysis (Microbe detection, ChIP DNA analysis)	Gene expression analysis (Microbe detection, ChIP DNA analysis)
Recommended master mix	PowerTrack SYBR Green Master Mix	PowerUpSYBR Green Master Mix
Specificity	Better	Best
Sensitivity	Best	Best
Pipetting tracking dye		No
Hot start	Antibody-mediated	Dual-lock for exceptional specificity
Cycling mode	Standard (~60 min) Fast (~30 min)	Standard (~60 min) Fast (~30 min)
Carryover contamination control	Includes heat-labile UNG and a blend of dTTP/dUTP	Includes heat-labile UNG and a blend of dTTP/dUTP
Instrument compatibility	Compatible with all Applied Biosystems real-time PCR instruments, Bio-Rad iQ™5 and CFX™ (96 and 384), and Roche LightCycler™ 480 systems	Compatible with all Applied Biosystems real-time PCR instruments, Bio-Rad iQ™5 and CFX™ (96 and 384), Roche LightCycler™ 480, and Stratagene Mx3005P™ systems
Recommended primer concentration	300–800 nM	300–800 nM
Recommended primer Tm	55–65°C	60°C
	Check price or order now Request a sample	Check price or order now

Looking for something else?

See the entire range of SYBR Green master mixes available from Thermo Fisher Scientific below.

Applied Biosystems products	Wide instrument compatibility, and the best choice for Applied Biosystems instruments. All mixes include ROX dye.
									Comments
PowerTrack SYBR Green Master Mix (Recommended)	•		•	•	••	•		•	Our newest and best mix. Designed to reduce pipetting errors via tracking dye. Formulated for excellent specificity, single-copy detection, and wide instrument compatibility.
PowerUp SYBR Green Master Mix	•	•	•	•	••	•	•		Formulated for exceptional specificity, single-copy detection, and wide instrument compatibility.
Power SYBR Green Master Mix (Best value)				•		•			Our most popular mix. A great value.
SYBR Select Master Mix	•		•	•	••	•			Our brightest mix. May be too bright for some older instruments.
Fast SYBR Green Master Mix			•	•	•	•			Our first mix formulated for fast cycling.
SYBR Green Master Mix				•		•			The original SYBR Green master mix.
MeltDoctor HRM Master Mix				•					Formulated specifically for high resolution melt (HRM) analysis.
Thermo Scientific products	Mixes optimized for specific instruments from Thermo Fisher Scientific and other suppliers. Versions with separate ROX, low ROX, high ROX, and fluorescein available.
Luminaris Color HiGreen qPCR Master Mix	•			•	•	V		•	Includes dye in mix and in sample buffer to aid pipetting.
Maxima SYBR Green qPCR Master Mix				•		V			A basic mix with great value, especially if you don't want premixed ROX.
DyNAmo ColorFlash SYBR Green qPCR Kit	•		•	•		V		•	A fast cycling–compatible mix that includes dye in mix and in sample buffer to aid pipetting.
Absolute QPCR Mix, SYBR Green						V			A basic mix, and the only one available without dUTP.
Invitrogen products	Mixes optimized for specific instruments from Thermo Fisher Scientific and other suppliers. Versions with separate ROX, low ROX, high ROX, and fluorescein available.
SYBR GreenER qPCR SuperMix				•	•	V			An updated, brighter version of Platinum SYBR Green supermix.
Express SYBR GreenER qPCR SuperMix			•	•	••	V			All the features of SYBR GreenER mix, but fast cycling–compatible. To be discontinued.
Platinum SYBR Green qPCR SuperMix-UDG				•	•	V			The original Invitrogen SYBR Green master mix.

1. • = master mix includes uracil N-glycosylase (UNG); •• = master mix includes a heat-labile form of UNG, which improves master mix performance compared to the standard UNG.
2. Master mix includes ROX, a passive reference dye; V = varies, different master mix versions are available with different amounts of ROX dye, or ROX included in a separate tube.